From wikipedia

At least some of the astronauts were likely alive and briefly conscious after the breakup, as three of the four Personal Egress Air Packs (PEAPs) on the flight deck were found to have been activated. Investigators found their remaining unused air supply roughly consistent with the expected consumption during the 2 minute 45 second post-breakup trajectory.

While analyzing the wreckage, investigators discovered that several electrical system switches on Pilot Mike Smith's right-hand panel had been moved from their usual launch positions. Fellow Astronaut Richard Mullane wrote, "These switches were protected with lever locks that required them to be pulled outward against a spring force before they could be moved to a new position." Later tests established that neither force of the explosion nor the impact with the ocean could have moved them, indicating that Smith made the switch changes, presumably in a futile attempt to restore electrical power to the cockpit after the crew cabin detached from the rest of the orbiter.[19]

Whether the astronauts remained conscious long after the breakup is unknown, and largely depends on whether the detached crew cabin maintained pressure integrity. If it did not, the time of useful consciousness at that altitude is just a few seconds; the PEAPs supplied only unpressurized air, and hence would not have helped the crew to retain consciousness. The cabin hit the ocean surface at roughly 207 mph (333 km/h), with an estimated deceleration at impact of well over 200 g, far beyond the structural limits of the crew compartment or crew survivability levels.[15]